1.Add reagent ka 100ml mmiri sample, mgbe dissolving, incubate na 36 ° C maka 24h
2. Nkọwa nke nsonaazụ:
enweghị agba = adịghị mma
Yellow = mma maka mkpokọta coliforms
Yellow + fluorescence = Escherichia coli ziri ezi.
QUANTlTATlVE DETECTlON
1. Tinye reagents na ihe nlele mmiri ma gwakọta nke ọma
2. Wụnye n'ime efere nchọpụta ọnụọgụ nke ọma 51 (ihe dị iche iche nke ọma) ma ọ bụ 97-nke ọma quantitative efere (quantitative well plate)
3. Jiri igwe akara ọnụọgụ nke na-achịkwa mmemme
iji mechie diski nchọpụta ọnụọgụ (quantitative well plate) maka akara na incubate na 36 ° C maka awa 24.
Omenala coliform/fecal coliform na-eguzogide okpomọkụ na 44.5°C maka awa 24 bụ edo edo ma dịkwa mma.
4. Nkọwa nke nsonaazụ:
enweghị agba = adịghị mma
Acha odo odo = ezigbo mkpokọta coliform
Yellow + fluorescent Grid = Escherichia coli ezigbo ntụaka MPN ọnụ ọgụgụ tebụl