Nchịkọta | A na-eji achọpụta ọrịa leukosis nke Avian P27 n'ime ọbara avian, feces, cloaca, na akwa ọcha. |
Ụkpụrụ | Avian Leukosis (AL) P27 antigen Elisa kit na-eji achọpụta Avian leukosis P27 antigen n'ime ọbara avian, feces, cloaca, na akwa ọcha.
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Ebumnuche nchọpụta | Ọrịa leukosis nke Avian (AL) antigen P27 |
Ihe atụ | Serum
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Ọnụọgụ | 1 ngwa = 192 Nnwale |
Nkwụsi ike na Nchekwa | 1) All reagents ga-echekwara na 2 ~ 8 ℃.Ajụla oyi. 2) Ndụ shelf bụ ọnwa 12.Jiri reagents niile tupu ụbọchị ngwụcha na ngwa ahụ.
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Avian Leukosis (AL) bụ okwu mkpokọta maka ọrịa dị iche iche metụtara tumor na anụ ọkụkọ nke Avian Leukosis Virus (ALV) kpatara na ezinụlọ Retroviridae.A na-ekesa ọrịa a n'ụwa niile ma nwee ọnụọgụ ọrịa dị elu.O nwere ike ime ka ọkụkọ na-anwụ anwụ na ahụ́ ọkụ, na-ebelata ikike imepụta atụrụ, ma bụrụkwa otu n'ime ọrịa ndị na-ebute mmepe nke ụlọ ọrụ anụ ọkụkọ n'ụzọ dị egwu.Ọrịa a nwere ogologo akụkọ ihe mere eme ma na-enwekarị ọhụụ ọhụrụ, dị ka ngalaba nje virus nke Avian leukemia J (ALV-J), bụ nke achọpụtara ma chọpụta na njedebe 1980 na UK dị ka ụdị ọhụrụ nke nje leukemia avian, na-eme ka ọ dị ukwuu. nsogbu na ụlọ ọrụ broiler
Ngwa ahụ na-eji usoro ELISA sanwichi, a na-ekpuchi ya nke ọma anti-avian leukocyte P27 monoclonal antibody na enzyme micro-well strips. N'ime ule ahụ, antigen dị na nlele ahụ na-ejikọta ya na mgbochi na efere mkpuchi, mgbe ịsachara iji wepụ. antigen na-enweghị njikọ na ihe ndị ọzọ, a na-agbakwunye antibody monoclonal enzyme na-ejikọta kpọmkwem na mgbagwoju antigen-antibody na efere ule.Mgbe ahụ, ịsa ahụ, wepụ conjugate enzyme na-enweghị njikọ, a na-agbakwunye ihe ngwọta TMB na microplate, mgbaàmà na-acha anụnụ anụnụ site na Enzyme catalysis bụ kpọmkwem nha nke ọdịnaya antibody na nlele.Tinye ngwọta nkwụsị, Mgbe mmeghachi omume gasịrị, a na-atụ uru nnabata A na mmeghachi omume nke ọma site na ogologo wavelength nke 450 nm.
Reagent | Olu Nnwale 96/Nnwale 192 | ||
1 |
| 1ea/2e | |
2 |
| 2.0ml | |
3 |
| 1.6 ml | |
4 |
| 100ml | |
5 |
| 100ml | |
6 |
| 11/22 ml | |
7 |
| 11/22 ml | |
8 |
| 15ml | |
9 |
| 2ea/4e | |
10 | microplate serum dilution | 1ea/2e | |
11 | Ntuziaka | 1 pcs |