Ngwaahịa
Akpụkpọ anụ Bovine Antibody ELISA Kit
Akpụkpọ anụ Bovine Antibody ELISA Kit
| Nchịkọta | Nchọpụta mgbochi ọrịa ụkwara nta Bovine (BTB). |
| Ụkpụrụ | Enwere ike iji ngwa nyocha nke Bovine Tuberculosis (BTB) iji chọpụta mgbochi ụkwara nta nke Bovine na serum ma ọ bụ plasma nke Bovine. |
| Ebumnuche nchọpụta | Ọgwụ mgbochi ọrịa ụkwara nta (BTB). |
| Ihe atụ | Serum
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| Ọnụọgụ | 1 ngwa = 192 Nnwale |
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Nkwụsi ike na Nchekwa | 1) All reagents ga-echekwara na 2 ~ 8 ℃.Ajụla oyi. 2) Ndụ shelf bụ ọnwa 12.Jiri reagents niile tupu ụbọchị ngwụcha na ngwa ahụ.
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Ozi
Mycobacterium bovis bụ nje na-eto ngwa ngwa (oge ọgbọ awa 16 ruo 20) nje nje na-akpata ụkwara nta na ehi (nke a maara dị ka TB bovine).Ọ metụtara Mycobacterium ụkwara nta, nje na-akpata ụkwara nta na ụmụ mmadụ.M. bovis nwere ike ima elu ihe mgbochi ụdị ma bute ọrịa ụkwara nta n'ime mmadụ na anụmanụ ndị ọzọ.
Ụkpụrụ nke ule
Nke a ngwa jiri indirect ELISA usoro, dị ọcha BTB antigen is tupu mkpuchi on enzyme micro-well ibe. Mgbe ị na-anwale, tinye diluted ọbara sample, emechaa incubation, if Ebe ahụ is BTB kpọmkwem antibody, it ga-eme ikpokọta ya na nke tupu mkpuchi antigen, tụfuo nke na-ejikọtaghị ọnụ ọgwụ mgbochi na ọzọ components ya na ịsa; mgbe ahụ tinye enzyme conjugate, tụfuo nke na-ejikọtaghị ọnụ enzyme conjugate ya na
ịsa ahụ. Tinye mkpụrụ osisi TMB n'ime obere olulu mmiri, mgbama na-acha anụnụ anụnụ sitere na Enzyme catalysis bụ ozugbo oke nke ọdịnaya mgbochi n'omume.
ọdịnaya
| Reagent | Olu Nnwale 96/Nnwale 192 | ||
| 1 |
| 1ea/2e | |
| 2 |
| 2.0ml | |
| 3 |
| 1.6 ml | |
| 4 |
| 100ml | |
| 5 |
| 100ml | |
| 6 |
| 11/22 ml | |
| 7 |
| 11/22 ml | |
| 8 |
| 15ml | |
| 9 |
| 2ea/4e | |
| 10 | microplate serum dilution | 1ea/2e | |
| 11 | Ntuziaka | 1 pcs |
