Nchịkọta | Nchọpụta mgbochi mgbochi nke Ụkwụ na Ọnụ Ọrịa Ụdị BRU |
Ụkpụrụ | Ngwa ELISA na-egbochi mgbochi BRU na-eji achọpụta ọgwụ mgbochi Brucellosis n'ime ọbara ezi, ehi, atụrụ na ewu. |
Ebumnuche nchọpụta | Ihe mgbochi BRU |
Ihe atụ | Serum
|
Ọnụọgụ | 1 ngwa = 192 Nnwale |
Nkwụsi ike na Nchekwa | 1) All reagents ga-echekwara na 2 ~ 8 ℃.Ajụla oyi. 2) Ndụ shelf bụ ọnwa 12.Jiri reagents niile tupu ụbọchị ngwụcha na ngwa ahụ.
|
Ọnụ ego BRU ọgwụ mgbochiELISAngwa ulejiri na- nchọpụta nke ọgwụ nje Brucellosis na serum nke ezì, ehi, sukwu na ewu .
Eji ngwa a usoro ELISA asọmpi maka uwe mkpuchinke BRU antigens na microplate olulu mmiri.Mgbe ị na-anwale, tinye ihe nlele serum agwara naEnzyme a na-akpọ anti-BRU monoclonal mgbochi, mgbe incubation, ọ bụrụ na e nwere nwere BRU mgbochi, ọ ga-ejikọta ya na antigen nke ekpuchiri mbụ, onye na-ahụ maka ihe mgbochi na-egbochi nchikota nke monoclonal antibody na antigen pre mkpuchi;tụfuo conjugate enzyme na-ejikọtaghị ọnụ na ịsacha;Tinye mkpụrụ osisi TMB n'ime obere olulu mmiri, akara na-acha anụnụ anụnụ site na Enzyme catalysis bụ n'ụzọ dị iche na ọdịnaya mgbochi mmadụ na nlele..
Reagent | Olu Nnwale 96/Nnwale 192 | ||
1 |
| 1ea/2e | |
2 |
| 2ml | |
3 |
| 1.6 ml | |
4 |
| 100ml | |
5 |
| 100ml | |
6 |
| 11/22 ml | |
7 |
| 11/22 ml | |
8 |
| 15ml | |
9 |
| 2ea/4e | |
10 | microplate serum dilution | 1ea/2e | |
11 | Ntuziaka | 1 pcs |