Nchịkọta | Nchọpụta mgbochi mgbochi nke Ụkwụ na Ọnụ Ọrịa Ụdị BRU |
Ụkpụrụ | Ụkpụrụ nke ule Brucellosis(Bru) antibody Elisa test kit nwere ike iji chọpụta ọgwụ mgbochi Brucellosis na serum ma ọ bụ plasma nke Bovine na Pig. |
Ebumnuche nchọpụta | Ihe mgbochi BRU |
Ihe atụ | Serum |
Ọnụọgụ | 1 ngwa = 192 Nnwale |
Nkwụsi ike na Nchekwa | 1) All reagents ga-echekwara na 2 ~ 8 ℃.Ajụla oyi. 2) Ndụ shelf bụ ọnwa 12.Jiri reagents niile tupu ụbọchị ngwụcha na ngwa ahụ.
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Nke a ngwa jiri indirect ELISA usoro, dị ọcha Bruantigen is tupu mkpuchi on enzyme micro-well ibe. Mgbe ị na-anwale, tinye diluted ọbara sample, emechaa incubation, if Ebe ahụ is Bru kpọmkwem antibody, it ga-eme ikpokọta ya na nke tupu mkpuchi antigen, tụfuo nke na-ejikọtaghị ọnụ ọgwụ mgbochi na ọzọ components ya na ịsa; mgbe ahụ tinye enzyme conjugate, tụfuo nke na-ejikọtaghị ọnụ enzyme conjugate ya na
ịsa ahụ. Tinye mkpụrụ osisi TMB n'ime obere olulu mmiri, mgbama na-acha anụnụ anụnụ sitere na Enzyme catalysis bụ ozugbo oke nke ọdịnaya mgbochi n'omume.
Reagent | Olu Nnwale 96/Nnwale 192 | ||
1 |
| 1ea/2e | |
2 |
| 2ml | |
3 |
| 1.6 ml | |
4 |
| 100ml | |
5 |
| 100ml | |
6 |
| 11/22 ml | |
7 |
| 11/22 ml | |
8 |
| 15ml | |
9 |
| 2ea/4e | |
10 | microplate serum dilution | 1ea/2e | |
11 | Ntuziaka | 1 pcs |