Nchịkọta | Uiji chọpụta ihe mgbochi pụrụ iche megide EDS76 na serum nke ọma. |
Ụkpụrụ | A na-eji ihe nje Drop Syndrome Eggs Drop Syndrome 1976 (EDS76) Ab Elisa kit chọpụta mgbochi mgbochi megide EDS76 na serum nke ọma.Maka nyochaa mgbochi mgbe EDS76 mgbochi ọrịa na serological diagnostic nke ọrịa na Avian. |
Ebumnuche nchọpụta | Aonye megidere EDS76 na serum |
Ihe atụ | Serum
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Ọnụọgụ | 1 ngwa = 192 Nnwale |
Nkwụsi ike na Nchekwa | 1) All reagents ga-echekwara na 2 ~ 8 ℃.Ajụla oyi. 2) Ndụ shelf bụ ọnwa 12.Jiri reagents niile tupu ụbọchị ngwụcha na ngwa ahụ.
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Egg Drop Syndrome (EDS-76) bụ nnukwu ọrịa na-efe efe nke adenovirus Group III nke Adenoviridae Avian Virus genus na hemagglutination kpatara.N'ubi ọkụkọ ụfọdụ, nnukwu anụ ọkụkọ a na-emepụta na-ebelata na mberede, na akwa ndị nwere nkwarụ dị ka akwa dị nro, akwa ndị na-adịchaghị obere, na akwa ndị dị obere ka a na-emepụta n'otu oge ahụ.Usoro niile nke ọrịa ahụ na-adịru izu 5-6, mgbe nke ahụ gasịrị, ọnụ ọgụgụ mmepụta akwa na-eji nwayọọ nwayọọ na-ebili, ma ọ na-esiri ike iru ọkwa ahụ tupu ọdịda.
Ngwa a na-eji usoro ELISA na-apụtaghị ìhè, EDS76 antigen dị ọcha bụ nke ekpuchiri ya na mpempe micro-well.,ọ bụrụ na e nwere EDS76virusspecificantibody, ọ ga-ejikọta ya na pre-coatedantigen, tụfuo antibody na ndị ọzọ na-asachapụ;tinyezie enzyme akpọrọ anti-EDS76 nje monoclonal antibody, wee ngwakọta nke mgbochi monoclonal na antigen pre mkpuchi;tụfuo conjugate enzyme na-ejikọtaghị ọnụ na ịsacha;Tinye TMB mkpụrụ n'ime micro-wells, akara na-acha anụnụ anụnụ site na Enzyme catalysis bụ n'ụzọ dị iche iche nke ọdịnaya antibody na sample, jiri ELISA agụ na 450nm wavelenth iji tụọ nnabata A uru na mmeghachi omume olulu mgbe gbakwunyere ngwọta nkwụsị.
ịkwụsị mmeghachi omume.
Reagent | Olu Nnwale 96/Nnwale 192 | ||
1 |
| 1ea/2e | |
2 |
| 2.0ml | |
3 |
| 1.6 ml | |
4 |
| 100ml | |
5 |
| 100ml | |
6 |
| 11/22 ml | |
7 |
| 11/22 ml | |
8 |
| 15ml | |
9 |
| 2ea/4e | |
10 | microplate serum dilution | 1ea/2e | |
11 | Ntuziaka | 1 pcs |