Nchịkọta | Nchọpụta nke kpọmkwemỌrịa Ụkwụ na Ọnụ Ụdị A ọgwụ mgbochi |
Ụkpụrụ | Ngwa ihe nyocha ELISA Ụdị FMD A na-eji achọpụta ọgwụ mgbochi ọrịa ụkwụ na ọnụ na serum nke ezì, ehi, atụrụ na ewu maka nyocha nke mgbochi mgbochi FMD. |
Ebumnuche nchọpụta | Ọrịa Ụkwụ na Ọnụ Ụdị A Antibody |
Ihe atụ | Serum
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Ọnụọgụ | 1 ngwa = 192 Nnwale |
Nkwụsi ike na Nchekwa | 1) All reagents ga-echekwara na 2 ~ 8 ℃.Ajụla oyi. 2) Ndụ shelf bụ ọnwa 12.Jiri reagents niile tupu ụbọchị ngwụcha na ngwa ahụ.
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Ọrịa ụkwụ na ọnụ(FMDV) bụ nke apathogennke na-akpataọrịa ụkwụ na ọnụ. Ọ bụ apicornavirus, prototypical so na genusAphthovirus.Ọrịa, nke na-akpata vesicles (blisters) n'ọnụ na ụkwụ nkeehi, ezi, atụrụ, ewu na ndị ọzọnke ukwu kpara akpaụmụ anụmanụ na-efe efe nke ukwuu na nnukwu ọrịaanụ ugbo.
Ngwa a na-eji usoro ELISA asọmpi na-ebute antigens ọrịa ụkwụ na ọnụ kpuchiri ya na olulu mmiri microplate.Mgbe ị na-anwale, gbakwunye ihe nlele serum diluted na mgbochi FMD Ab, mgbe mmechi ahụ gasịrị, ọ bụrụ na enwere FMD antibody, ọ ga-ejikọta ya na antigen pre mkpuchi, mgbochi na sample na-egbochi nchikota nke anti-FMD antibody na pre mkpuchi antigen;tụfuo conjugate enzyme na-ejikọtaghị ọnụ na ịsacha;Tinye mkpụrụ osisi TMB n'ime obere olulu mmiri, mgbama na-acha anụnụ anụnụ site na Enzyme catalysis bụ n'oke nha nke ọdịnaya mgbochi mmadụ na nlele.
Ọrịa ụkwụ na ọnụemee na isi asaaserotypes: O, A, C, SAT-1, SAT-2, SAT-3, na Asia-1.Ndị a serotypes na-egosi ụfọdụ mpaghara, na O serotype bụ ihe a na-ahụkarị.
Reagent | Olu Nnwale 96/Nnwale 192 | ||
1 |
| 1ea/2e | |
2 |
| 2.0ml | |
3 |
| 1.6 ml | |
4 |
| 100ml | |
5 |
| 100ml | |
6 |
| 11/22 ml | |
7 |
| 11/22 ml | |
8 |
| 15ml | |
9 |
| 2ea/4e | |
10 | microplate serum dilution | 1ea/2e | |
11 | Ntuziaka | 1 pcs |