Ngwaahịa
Ụdị Ọrịa Ụkwụ na Ọnụ O Ab ELISA
Ụdị Ọrịa Ụkwụ na Ọnụ O Ab ELISA
| Nchịkọta | Nchọpụta nke kpọmkwemỤkwụ na Ọnụ ọgwụ mgbochiỤdị O |
| Ụkpụrụ | Ngwa FMD Type Asia I antibody ELISA na-eji achọpụta ọgwụ mgbochi ọrịa ụkwụ na ọnụ na serum nke ezì, ehi, atụrụ na ewu maka nyocha nke mgbochi mgbochi FMD. |
| Ebumnuche nchọpụta | Ụkwụ na Ọnụ ọgwụ mgbochiỤdị O |
| Ihe atụ | Serum
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| Ọnụọgụ | 1 ngwa = 192 Nnwale |
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Nkwụsi ike na Nchekwa | 1) All reagents ga-echekwara na 2 ~ 8 ℃.Ajụla oyi. 2) Ndụ shelf bụ ọnwa 12.Jiri reagents niile tupu ụbọchị ngwụcha na ngwa ahụ.
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Ozi
Ọrịa ụkwụ na ọnụ(FMDV) bụ nke apathogennke na-akpataọrịa ụkwụ na ọnụ.[1]Ọ bụ apicornavirus, prototypical so na genusAphthovirus.Ọrịa, nke na-akpata vesicles (blisters) n'ọnụ na ụkwụ nkeehi, ezi, atụrụ, ewu na ndị ọzọnke ukwu kpara akpaụmụ anụmanụ na-efe efe nke ukwuu na nnukwu ọrịaanụ ugbo
Ụkpụrụ nke ule
Nke a na usoro ELISA na-eduzi, FMDVantigenis kpuchiri ya na ibe micro-well.Mgbe a na-anwale, agbakwunyere serum sample, mgbe incubation gasịrị, ọ bụrụ na enwere nje nje FMD kpọmkwem, ọ ga-ejikọta ya na antigen nke ekpuchiri mbụ, tụfuo ihe mgbochi na-ejikọtaghị ọnụ na ihe ndị ọzọ na ịsacha;wee tinye enzyme conjugate, tụfuo conjugate enzyme na-ejikọtaghị ya na ịsacha.Tinye TMB mkpụrụ n'ime obere olulu mmiri, mgbama na-acha anụnụ anụnụ site na Enzyme catalysis bụ oke nke ọdịnaya mgbochi mmadụ na nlele.
ọdịnaya
| Reagent | Olu Nnwale 96/Nnwale 192 | ||
| 1 |
| 1ea/2e | |
| 2 |
| 2ml | |
| 3 |
| 1.6 ml | |
| 4 |
| 100ml | |
| 5 |
| 100ml | |
| 6 |
| 11/22 ml | |
| 7 |
| 11/22 ml | |
| 8 |
| 15ml | |
| 9 |
| 2ea/4e | |
| 10 | microplate serum dilution | 1ea/2e | |
| 11 | Ntuziaka | 1 pcs |
