Nchịkọta | Nchọpụta ihe mgbochi ọrịa flu Nchọpụta ihe mgbochi na serological nke ọrịa na Avian, ezì na Equus. |
Ụkpụrụ | Influenza A na-eji ihe mgbochi Elisa kit chọpụta ihe mgbochi pụrụ iche megide INje Virus (Flu A) na serum, maka nlekota mgbochi ọrịa mgbe flu Ihe mgbochina serological nchoputa nke ọrịa na Avian, ezì na Equus.
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Ebumnuche nchọpụta | Influenza A mgbochi |
Ihe atụ | Serum
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Ọnụọgụ | 1 ngwa = 192 Nnwale |
Nkwụsi ike na Nchekwa | 1) All reagents ga-echekwara na 2 ~ 8 ℃.Ajụla oyi. 2) Ndụ shelf bụ ọnwa 12.Jiri reagents niile tupu ụbọchị ngwụcha na ngwa ahụ.
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Influenza A bụ nje na-akpata influenza na nnụnụ na ụfọdụ anụ mamma.Ọ bụ nje RNA, nke ụdị nke dị n'ụdị ya dịpụrụ adịpụ na nnụnụ ọhịa.Mgbe ụfọdụ, ọ na-agbasa site na nnụnụ ọhịa gaa n'anụ ọkụkọ, nke nwere ike ibute ọrịa siri ike, ntiwapụ, ma ọ bụ ọrịa influenza mmadụ.
Ngwa a na-eji ụzọ mgbochi ELISA, FluA antigen bụ ihe mkpuchi ya na microplate.Mgbe a na-anwale ya, tinye ihe nlele serum agwara, ka emechara ya, ọ bụrụ na enwere flu Otu mgbochi kpọmkwem, ọ ga-ejikọta ya na antigen nke ekpuchiri mbụ, tụfuo ihe mgbochi na-ejikọtaghị ya na ihe ndị ọzọ na-asachapụ;wee tinye enzyme akpọrọ mgbochi flu Ihe mgbochi monoclonal, onye na-ahụ maka ihe nlere anya na-egbochi nchikota nke mgbochi monoclonal na antigen nke mkpuchi mkpuchi;tụfuo enzyme na-ejikọtaghị ọnụ na ịsacha. Tinye mkpụrụ osisi TMB n'ime obere olulu mmiri, mgbama na-acha anụnụ anụnụ site na Enzyme catalysis bụ n'oke nha nke ọdịnaya mgbochi mmadụ na nlele.
Reagent | Olu Nnwale 96/Nnwale 192 | ||
1 |
| 1ea/2e | |
2 |
| 2.0ml | |
3 |
| 1.6 ml | |
4 |
| 100ml | |
5 |
| 100ml | |
6 |
| 11/22 ml | |
7 |
| 11/22 ml | |
8 |
| 15ml | |
9 |
| 2ea/4e | |
10 | microplate serum dilution | 1ea/2e | |
11 | Ntuziaka | 1 pcs |