Nchịkọta | Nchọpụta mgbochi mgbochi ọrịa Newcastle kpọmkwem ọgwụ mgbochi ọrịa |
Ụkpụrụ | A na-eji ngwa mgbochi ọrịa Newcastle Elisa kit chọpụta ọgwụ mgbochi pụrụ iche megide ọrịa Newcastle Nje Virus (NDV) na serum, maka nyochaa mgbochi mgbe NDV nwesịrịna nchọpụta serological nke ọrịa na Avian.
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Ebumnuche nchọpụta | Ọgwụ mgbochi ọrịa Newcastle |
Ihe atụ | Serum
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Ọnụọgụ | 1 ngwa = 192 Nnwale |
Nkwụsi ike na Nchekwa | 1) All reagents ga-echekwara na 2 ~ 8 ℃.Ajụla oyi. 2) Ndụ shelf bụ ọnwa 12.Jiri reagents niile tupu ụbọchị ngwụcha na ngwa ahụ.
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Ọrịa Newcastle bụ ọrịa avian na-efe efe na-efe efe na-emetụta ọtụtụ ụdị nnụnụ anụ ụlọ na nke anụ ọhịa;ọ na-ebufe mmadụ. Ọ bụ ezie na ọ nwere ike ibunye mmadụ, ọtụtụ ikpe na-abụghị nke mgbaàmà;adịkarịghị, ọ nwere ike ibute obere ahụ ọkụ na influenza dị ka ihe mgbaàmà na/ma ọ bụ conjunctivitis n'ime mmadụ.Mmetụta ya bụ ihe a ma ama na anụ ọkụkọ ụlọ n'ihi oke ike ha na ike inwe mmetụta siri ike nke epizootic na ụlọ ọrụ anụ ọkụkọ.Ọ na-efe efe n'ọtụtụ mba.Enweghị ọgwụgwọ maka ya mara, mana iji ọgwụ mgbochi prophylactic na usoro ịdị ọcha na-ebelata ohere nke ntiwapụ.
Ngwa a na-eji ụzọ mgbochi ELISA, NDV antigen bụ nke ekpuchiri mbụ na microplate.Mgbe ị na-anwale, gbakwunye ihe nlele serum diluted, mgbe a mụsịrị ya, ọ bụrụ na enwere mgbochi NDV kpọmkwem, ọ ga-ejikọta ya na antigen nke kpuchiri ekpuchi mbụ, tụfuo ihe mgbochi na-ejikọtaghị ya na ihe ndị ọzọ na ịsacha;wee tinye enzyme akpọrọ anti-NDV monoclonal antibody, mgbochi na sample igbochi nchikota nke monoclonal mgbochi na antigen pre mkpuchi;tụfuo enzyme na-ejikọtaghị ọnụ na ịsacha. Tinye mkpụrụ osisi TMB n'ime obere olulu mmiri, mgbama na-acha anụnụ anụnụ site na Enzyme catalysis bụ n'oke nha nke ọdịnaya mgbochi mmadụ na nlele.
Reagent | Olu Nnwale 96/Nnwale 192 | ||
1 |
| 1ea/2e | |
2 |
| 2.0ml | |
3 |
| 1.6 ml | |
4 |
| 100ml | |
5 |
| 100ml | |
6 |
| 11/22 ml | |
7 |
| 11/22 ml | |
8 |
| 15ml | |
9 |
| 2ea/4e | |
10 | microplate serum dilution | 1ea/2e | |
11 | Ntuziaka | 1 pcs |