Ngwaahịa
Peste Des Petits Ruminants Ab ELISA Kit
Peste Des Petits Ruminants Ab ELISA Kit
| Nchịkọta | Nchọpụta kpọmkwem Antibody nke Peste Des Petits Ruminants |
| Ụkpụrụ | Ngwa nyocha nke ELISA nke PPRV na-eji achọpụta nje nje nje Peste des petits n'ime ọbara atụrụ na ewu. |
| Ebumnuche nchọpụta | Ihe mgbochi PPV |
| Ihe atụ | Serum
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| Ọnụọgụ | 1 ngwa = 192 Nnwale |
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Nkwụsi ike na Nchekwa | 1) All reagents ga-echekwara na 2 ~ 8 ℃. Ajụla oyi. 2) Ndụ shelf bụ ọnwa 12. Jiri reagents niile tupu ụbọchị ngwụcha na ngwa ahụ.
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Ozi
Ovine rinderpest, nke a na-akpọkarịpeste des petits ruminants(PPR), bụ ọrịa na-efe efe na-ebute ụzọewunaatụrụ; otu o sila dị, kamel na anụ ọhịa ntaruminantsnwekwara ike imetụta. PPR dị ugbu a naUgwu, Central, WestnaEast Africa, nkeEtiti ọwụwa anyanwụ, naSouth Asia. Ihe kpatara yaobere mịrị amị morbillivirusna genusỌrịa Morbilli,na ihe jikọrọ ya na ndị ọzọ. ọrịa nje morbilli, ọrịa measles morbillivirus, naọrịa nje morbilli(nke a na-akpọbucaninenje virus distemper). Ọrịa a na-efe efe nke ukwuu, ma nwee ike ịnwe ọnụọgụ ọnwụ 80-100%.nnukwuikpe na aepizooticntọala. Nje virus anaghị ebute mmadụ.
A na-akpọkwa PPR dị ka ọrịa ewu,kata, ọrịa stomatitis-pneumoenteritis, na ovine rinderpest.
Ụlọ ọrụ gọọmentị dị kaFAOnaOIEjiri aha French"peste des petits ruminants"nwere ọtụtụ ụdị mkpụrụ okwu.
Ụkpụrụ nke ule
Ngwa a na-eji usoro ELISA asọmpi emechie antigens PPRV na olulu mmiri microplate. Mgbe ị na-anwale, gbakwunye ihe nlele serum diluted, mgbe a mụsịrị ya, ọ bụrụ na enwere ọgwụ mgbochi PPRV, ọ ga-ejikọta ya na antigen nke kpuchiri mbụ, mgbochi na-egbochi nchikota nke monoclonal antibody na antigen pre mkpuchi; tụfuo conjugate enzyme na-ejikọtaghị ọnụ na ịsacha; Tinye mkpụrụ osisi TMB n'ime obere olulu mmiri, mgbama na-acha anụnụ anụnụ site na Enzyme catalysis bụ n'oke nha nke ọdịnaya mgbochi mmadụ na nlele.
ọdịnaya
| Reagent | Olu Nnwale 96/Nnwale 192 | ||
| 1 |
| 1ea/2e | |
| 2 |
| 2ml | |
| 3 |
| 1.6 ml | |
| 4 |
| 100ml | |
| 5 |
| 100ml | |
| 6 |
| 11/22 ml | |
| 7 |
| 11/22 ml | |
| 8 |
| 15ml | |
| 9 |
| 2ea/4e | |
| 10 | microplate serum dilution | 1ea/2e | |
| 11 | Ntuziaka | 1 pcs |
