Ngwaahịa
Ihe mgbochi ọrịa influenza ELISA
Ihe mgbochi ọrịa influenza ELISA
| Nchịkọta | A na-eji ya chọpụta ọgwụ mgbochi ụfọdụ megide nje influenza avian (AIV) na serum |
| Ụkpụrụ | A na-eji ihe mgbochi influenza nke Avian Elisa kit chọpụta ihe mgbochi pụrụ iche megide nje virus influenza Avian (AIV) na serum, maka nlekota mgbochi ọrịa mgbe AIV nwesịrị na nchọpụta serological nke ọrịa na Avian.. |
| Ebumnuche nchọpụta | Antibody nke influenza |
| Ihe atụ | Serum
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| Ọnụọgụ | 1 ngwa = 192 Nnwale |
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Nkwụsi ike na Nchekwa | 1) All reagents ga-echekwara na 2 ~ 8 ℃.Ajụla oyi. 2) Ndụ shelf bụ ọnwa 12.Jiri reagents niile tupu ụbọchị ngwụcha na ngwa ahụ.
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Ozi
Influenza Avian, nke a na-akpọkarị dị ka flu avian ma ọ bụ flu nnụnụ, bụ ụdị influenza dị iche iche nke nje na-eme ka ọ dị.nnụnụ.
Ụdị nwere nnukwu ihe ize ndụ bụ nnukwu nje influenza avian (HPAI).Ọrịa nnụnụ yiri yaflu ezì, nkịta flu, inyinya
flu na flu mmadụ dị ka ọrịa nke ụdị nje nje influenza na-eme ka ọ bụrụ nke dabara n'otu n'otu
onye ọbịa.N'ime ụdị nje influenza atọ (A,B, naC), nje influenza A bụ azoonoticọrịa na a eke
mmiri mmiri fọrọ nke nta ka ọ bụrụ na nnụnụ.Influenza Avian, maka ọtụtụ ebumnuche, na-ezo aka na nje influenza A.
Ụkpụrụ nke ule
Ngwa a na-eji ụzọ ELISA egbochi, AIV antigen bụ ihe mkpuchi ya na microplate.Mgbe ị na-anwale, gbakwunye ihe nlele serum diluted, mgbe a kpọchara ya, ọ bụrụ na e nwere mgbochi AIV kpọmkwem, ọ ga-ejikọta ya na antigen a na-ekpuchi ya, tụfuo ihe mgbochi na-ejikọtaghị ya na ihe ndị ọzọ na ịsacha;wee tinye enzyme akpọrọ anti-AIV monoclonal antibody, mgbochi na sample igbochi Nchikota monoclonal antigen na tupu mkpuchi antigen;tụfuo enzyme na-ejikọtaghị ọnụ na ịsacha.Tinye mkpụrụ osisi TMB n'ime obere olulu mmiri, mgbama na-acha anụnụ anụnụ site na Enzyme catalysis bụ n'oke nha nke ọdịnaya mgbochi mmadụ na nlele.
ọdịnaya
| Reagent | Olu Nnwale 96/Nnwale 192 | ||
| 1 |
| 1ea/2e | |
| 2 |
| 2.0ml | |
| 3 |
| 1.6 ml | |
| 4 |
| 100ml | |
| 5 |
| 100ml | |
| 6 |
| 11/22 ml | |
| 7 |
| 11/22 ml | |
| 8 |
| 15ml | |
| 9 |
| 2ea/4e | |
| 10 | microplate serum dilution | 1ea/2e | |
| 11 | Ntuziaka | 1 pcs |
