Nchịkọta | Nchọpụta nke na-ewepụ ihe mgbochi megide nje virus Fabricius na-efe efe na serum ọkụkọ |
Ebumnuche nchọpụta | Ihe mgbochi ọrịa bursal ọrịa ọkụkọ |
Ihe atụ | Serum
|
Ọnụọgụ | 1 ngwa = 192 Nnwale |
Nkwụsi ike na Nchekwa | 1) All reagents ga-echekwara na 2 ~ 8 ℃.Ajụla oyi. 2) Ndụ shelf bụ ọnwa 12.Jiri reagents niile tupu ụbọchị ngwụcha na ngwa ahụ.
|
Ọrịa bursal na-efe efe(IBD), nke a makwaara dị ka ọrịa Gumboro, ọrịa bursitis na-efe efe na avian nephrosis na-efe efe, bụ ọrịa na-efe efe nke ukwuu nke ndị na-eto eto.ọkụkọna turkey nke nje ọrịa bursal na-efe efe (IBDV) kpatara, nke ejiri mara yaimmunosuppressionna ọnwụ n'ozuzu mgbe ọ dị izu 3 ruo 6.Achọpụtara ọrịa ahụ na mbụGumboro, Delawarena 1962. Ọ dị mkpa akụ na ụba na ụlọ ọrụ anụ ọkụkọ n'ụwa nile n'ihi mmụba nke ọrịa ndị ọzọ na nnyonye anya na-adịghị mma na-arụ ọrụ nke ọma.ịgba ọgwụ mgbochi ọrịa.N'afọ ndị na-adịbeghị anya, ụdị ọrịa IBDV (vvIBDV) na-efe efe, na-ebute oke ọnwụ na ọkụkọ, apụtala na Europe.Latin America,South-East Asia, Africa na ndịEtiti ọwụwa anyanwụ.Ọrịa na-esite n'ụzọ oro-fecal, na nnụnụ ndị metụtara na-ewepụta oke nje ahụ ruo ihe dị ka izu abụọ mgbe ọrịa ahụ gasịrị.A na-esi n'ọkụkọ ndị bu ọrịa bute ọrịa ahụ n'ụzọ dị mfe site na nri, mmiri, na mmetụ ahụ́.
Ngwa ahụ na-eji usoro ELISA asọmpi, eburu ụzọ chịkọta ọrịa bursal na-efe efe VP2 protein na microplate, wee soro anti-VP2 protein antibody na serum maka vector nke siri ike na-eji mgbochi VP2 protein monoclonal antibody.N'ime ule ahụ, a ga-anwale ihe mgbochi monoclonal na-agbakwunye protein mgbochi VP2, na mgbe a na-emepụta ya, ọ bụrụ na ihe nlele ahụ nwere ọrịa ọrịa bursal na-efe efe nke ọkụkọ VP2, ọ na-ejikọta na antigen na efere mkpuchi.Site na nke a na-egbochi njikọ nke anti-VP2 protein monoclonal antibody na antigen, mgbe ịsachara iji wepụ ihe mgbochi na-enweghị njikọ na ihe ndị ọzọ;wee gbakwunye mgbochi mgbochi òké enzyme nke akpọrọ ihe mgbochi nke abụọ iji jikọta kpọmkwem na mgbagwoju antigen-antibody na efere nchọpụta;A na-ewepụ conjugate enzyme a na-ekekọta site na ịsacha;A na-agbakwunye mkpụrụ TMB na microwell iji mepụta agba, yana uru nnabata nke ihe nlele ahụ na-ejikọta ya na ọdịnaya nke anti-VP2 protein antibody dị n'ime ya, si otú a na-enweta nzube nke ịchọpụta anti-VP2 protein antibody na sample.
Reagent | Olu Nnwale 96/Nnwale 192 | ||
1 |
| 1ea/2e | |
2 |
| 2.0ml | |
3 |
| 1.6 ml | |
4 |
| 100ml | |
5 |
| 100ml | |
6 |
| 11/22 ml | |
7 |
| 11/22 ml | |
8 |
| 15ml | |
9 |
| 2ea/4e | |
10 | microplate serum dilution | 1ea/2e | |
11 | Ntuziaka | 1 pcs |