Ngwaahịa-ọkọlọtọ

Ngwaahịa

Ọrịa Bursal Ọrịa Chicken na-efe efe Ab Elisa ngwa

Koodu ngwaahịa:

Aha ihe: Chicken Infection Bursal Disease Virus Ab Elisa kit

Nchịkọta: Ngwa ihe nchọpụta ọrịa na-efe efe nke ọkụkọ na-efe efe nke ọrịa bursal ọrịa nke na-ewepụ mgbochi mgbochi na serum ọkụkọ maka nyochaa mgbochi mgbochi nke ọkụkọ na-efe efe ọrịa mgbochi ọrịa mgbochi ọrịa na-ebute ọnọdụ yana enyemaka serologically nyere aka nyocha nke ọkụkọ butere.

Ebumnuche nchọpụta: ọrịa nje na-efe efe nke ọkụkọ

Nlele ule: Serum

Nkọwa: 1 kit = 192 Ule

Nchekwa: All reagents ga-echekwara na 2 ~ 8 ℃.Ajụla oyi.

Oge nchekwa: ọnwa iri na abụọ.Jiri reagents niile tupu ụbọchị ngwụcha na ngwa ahụ.


Nkọwa ngwaahịa

Mkpado ngwaahịa

Ọrịa Bursal Ọrịa Chicken na-efe efe Ab Elisa ngwa

Nchịkọta  Nchọpụta nke na-ewepụ ihe mgbochi megide nje virus Fabricius na-efe efe na serum ọkụkọ
Ebumnuche nchọpụta Ihe mgbochi ọrịa bursal ọrịa ọkụkọ
Ihe atụ Serum

 

Ọnụọgụ 1 ngwa = 192 Nnwale
 

 

Nkwụsi ike na Nchekwa

1) All reagents ga-echekwara na 2 ~ 8 ℃.Ajụla oyi.

2) Ndụ shelf bụ ọnwa 12.Jiri reagents niile tupu ụbọchị ngwụcha na ngwa ahụ.

 

 

 

Ozi

Ọrịa bursal na-efe efe(IBD), nke a makwaara dị ka ọrịa Gumboro, ọrịa bursitis na-efe efe na avian nephrosis na-efe efe, bụ ọrịa na-efe efe nke ukwuu nke ndị na-eto eto.ọkụkọna turkey nke nje ọrịa bursal na-efe efe (IBDV) kpatara, nke ejiri mara yaimmunosuppressionna ọnwụ n'ozuzu mgbe ọ dị izu 3 ruo 6.Achọpụtara ọrịa ahụ na mbụGumboro, Delawarena 1962. Ọ dị mkpa akụ na ụba na ụlọ ọrụ anụ ọkụkọ n'ụwa nile n'ihi mmụba nke ọrịa ndị ọzọ na nnyonye anya na-adịghị mma na-arụ ọrụ nke ọma.ịgba ọgwụ mgbochi ọrịa.N'afọ ndị na-adịbeghị anya, ụdị ọrịa IBDV (vvIBDV) na-efe efe, na-ebute oke ọnwụ na ọkụkọ, apụtala na Europe.Latin America,South-East Asia, Africa na ndịEtiti ọwụwa anyanwụ.Ọrịa na-esite n'ụzọ oro-fecal, na nnụnụ ndị metụtara na-ewepụta oke nje ahụ ruo ihe dị ka izu abụọ mgbe ọrịa ahụ gasịrị.A na-esi n'ọkụkọ ndị bu ọrịa bute ọrịa ahụ n'ụzọ dị mfe site na nri, mmiri, na mmetụ ahụ́.

Ụkpụrụ nke ule

Ngwa ahụ na-eji usoro ELISA asọmpi, eburu ụzọ chịkọta ọrịa bursal na-efe efe VP2 protein na microplate, wee soro anti-VP2 protein antibody na serum maka vector nke siri ike na-eji mgbochi VP2 protein monoclonal antibody.N'ime ule ahụ, a ga-anwale ihe mgbochi monoclonal na-agbakwunye protein mgbochi VP2, na mgbe a na-emepụta ya, ọ bụrụ na ihe nlele ahụ nwere ọrịa ọrịa bursal na-efe efe nke ọkụkọ VP2, ọ na-ejikọta na antigen na efere mkpuchi.Site na nke a na-egbochi njikọ nke anti-VP2 protein monoclonal antibody na antigen, mgbe ịsachara iji wepụ ihe mgbochi na-enweghị njikọ na ihe ndị ọzọ;wee gbakwunye mgbochi mgbochi òké enzyme nke akpọrọ ihe mgbochi nke abụọ iji jikọta kpọmkwem na mgbagwoju antigen-antibody na efere nchọpụta;A na-ewepụ conjugate enzyme a na-ekekọta site na ịsacha;A na-agbakwunye mkpụrụ TMB na microwell iji mepụta agba, yana uru nnabata nke ihe nlele ahụ na-ejikọta ya na ọdịnaya nke anti-VP2 protein antibody dị n'ime ya, si otú a na-enweta nzube nke ịchọpụta anti-VP2 protein antibody na sample.

ọdịnaya

 

Reagent

Olu

Nnwale 96/Nnwale 192

1
Microplate mkpuchi antigen

 

1ea/2e

2
 Njikwa adịghị mma

 

2.0ml

3
 Njikwa ziri ezi

 

1.6 ml

4
 Ihe atụ diluents

 

100ml

5
Ngwọta ịsa (10X gbadoro anya)

 

100ml

6
 Enzyme conjugate

 

11/22 ml

7
 Mkpụrụ

 

11/22 ml

8
 Nkwụsị ngwọta

 

15ml

9
Ihe mkpuchi efere nrapado

 

2ea/4e

10 microplate serum dilution

1ea/2e

11  Ntuziaka

1 pcs

 


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