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Teknụzụ nchọpụta Enzvme nke Enterococcus Maka nnwale mmiri egosipụtara
  • Teknụzụ nchọpụta Enzvme nke Enterococcus Maka nnwale mmiri
  • Teknụzụ nchọpụta Enzvme nke Enterococcus Maka nnwale mmiri

Teknụzụ nchọpụta Enzvme nke Enterococcus Maka nnwale mmiri

Koodu ngwaahịa:

Aha ihe: Enzvme nchọpụta technolouv nke Enterococcus

Character: Ngwaahịa a dị ọcha ma ọ bụ ihe na-acha edo edo edo edo edo edo

Enweghị agba: ma ọ bụ edo edo dị ọkụ

pH 7.0-7.6

Arọ: 2.7 士 0.5g

Nchekwa: Nchekwa na 4 ° C - 8 ° C, N'ebe dị jụụ ma chebe ọkụ

Ndaba: 1 Afọ, Lee nkwakọ ngwaahịa reagent maka ụbọchị mmepụta na ụbọchị ngafe.

Sayensị: Tinye ihe nlele mmiri nwere nje bacteria Enterococcus, mebere nje bacteria lekwasịrị anya na Mug na 41 Celsius C na 0.5 Celsius C, yana enzymes pụrụ iche nke nje bacteria Enterococcus mepụtara (3-0-gluco sidase nwere ike decompose

fluorescent substrate iko n'ime iko iko imepụta (3-D-glucoside ((3-0-glucoside) na

njirimara fluorescent ngwaahịa 4-methyl umbelliferone.Lelee fluorescence na oriọna UV 366nm, gụọ diski nchọta ọnụọgụ, wee jụọ tebụl MPN ka ịgbakọ nsonaazụ ya.

Ngwugwu: 100 - ngwugwu ule


Katalọgụ

Nkọwa ngwaahịa

Mkpado ngwaahịa

agba 1
asdasd2
agba3

agwa

 Ngwaahịa a bụ ihe na-acha ọcha ma ọ bụ ìhè edo edo

Ido anya

 Agba na-enweghị agba ma ọ bụ edo edo

PH

 7.0-7.6

Wasatọ

 2.7 mk 0.5g

Nchekwa

 na 4 ° C - 8 Celsius C, na ebe dị jụụ ma chebe ọkụ

Nkwenye

 Afọ 1, Lee nkwakọ ngwaahịa reagent maka ụbọchị mmepụta na ụbọchị ngafe.

Sayensị

 Tinye ihe nlele mmiri nwere nje bacteria Enterococcus, mebere nje bacteria lekwasịrị anya na Mug ọkara na 41ºC na 0.5ºC, yana enzymes bological nke nje bacteria Enterococcus mepụtara (β-D-glucosidase nwere ike imebi ihe mkpuchi fluorescent n'ime nnukwu iko iji mepụta. (β-D-glucoside ((β-D-glucoside) na njirimara fluorescent ngwaahịa 4-methyl umbelliferone. Leba anya na fluorescence na oriọna 366nm UV, gụọ site na diski ọnụọgụ ọnụọgụ, wee jụọ tebụl MPN iji gbakọọ nsonaazụ.

ngwugwu

 100 - mkpọ ule

Usoro ojiji Nnwale qualitative

Setp1: Were 100ml mmiri sample na 100ml sterile sampling karama / quantitative karama, tinye reagent, igbari, na omenala maka 24h na 41 ° C 0 .5°C.

Setp2: Mkpebi nsonaazụ

Enweghị fluorescence = arụpụtaghị ihe na-adịghị mma Blue fluorescence = ezigbo nsonaazụ

Nchọpụta ọnụọgụgụ

1.Use 100ml sterile sampling karama tụọ 100ml mmiri sample.

2.Add reagent, igbari ya, wunye ya n'ime 51 quanti tray ma ọ bụ 97 quanti-Tray

3.Package na Quanti-Tray na ihe omume na-achịkwa quantitative sealing igwe, na incubate ya na 41°C 士 0 .5 Celsius C maka 24h.

4. Rụtụ aka na ule qualitative maka nkọwa nsonaazụ, ma lelee tebụl MPN maka ịgụta.

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