Nchịkọta | Nchọpụta Ọrịa Ọrịa Hydatid |
Ụkpụrụ | Enwere ike iji ngwa nyocha Elisa chọpụta ihe mgbochi ọrịa Hydatid n'ime ọbara nke ehi, ewu na atụrụ. |
Ebumnuche nchọpụta | Ọrịa Hydatid Antibody |
Ihe atụ | Serum
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Ọnụọgụ | 1 ngwa = 192 Nnwale |
Nkwụsi ike na Nchekwa | 1) All reagents ga-echekwara na 2 ~ 8 ℃.Ajụla oyi. 2) Ndụ shelf bụ ọnwa 12.Jiri reagents niile tupu ụbọchị ngwụcha na ngwa ahụ.
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A makwaara dị ka ọrịa hydatid, ọ bụ ọrịa nje nwere ike imetụta mmadụ na anụmanụ ndị ọzọ dị ka atụrụ, nkịta, oke na ịnyịnya.A na-ahụ ụdị echinococcosis atọ dị iche iche n'ime mmadụ, nke ọ bụla na-akpata site na larvae nke ụdị dị iche iche Echinococcus granulosus tapeworm.Nke mbụ n'ime ọrịa ndị a na-ahụ na ụmụ mmadụ bụ cystic echinococcosis (nke a makwaara dị ka cystic echinococcosis), nke Echinococcus granulosus (aha sayensị: Echinococcus granulosus) kpatara.Ebe nke abụọ bụ alveolar echinococcosis (nke a makwaara dị ka alveolar echinococcosis), bụ nke echinococcosis follicular kpatara (aha sayensị: Echinococcus multilocularis).Mgbe mmalite ahụ gasịrị, mgbaàmà na akara onye ọrịa na-adabere na ebe na nha echinococcosis.Echinococcosis alveolar na-amalitekarị n'ime imeju, mana enwere ike gbasaa na saịtị ndị ọzọ, dị ka ngụgụ na ụbụrụ.Mgbe ọnya imeju na-etolite, ihe ịrịba ama ụlọ ọgwụ nke ndị ọrịa nwere ike ịgụnye mgbu afọ, oke ibu, na jaundice.Ọnya akpa ume nwere ike ịkpata mgbu obi, mkpụmkpụ ume, na ụkwara
Nke a ngwa jiri indirect ELISA usoro, dị ọcha HYD antigen is tupu mkpuchi on enzyme micro-well ibe. Mgbe ị na-anwale, tinye diluted ọbara sample, emechaa incubation, if Ebe ahụ is HYD nje kpọmkwem antibody, it ga-eme ikpokọta ya na nke tupu mkpuchi antigen, tụfuo nke na-ejikọtaghị ọnụ ọgwụ mgbochi na ọzọ components ya na ịsa; mgbe ahụ tinye enzyme conjugate, tụfuo nke na-ejikọtaghị ọnụ enzyme conjugate na ịsa ahụ. Tinye mkpụrụ osisi TMB n'ime obere olulu mmiri, mgbama na-acha anụnụ anụnụ sitere na Enzyme catalysis bụ ozugbo oke nke ọdịnaya mgbochi n'omume.
Reagent | Olu Nnwale 96/Nnwale 192 | ||
1 |
| 1ea/2e | |
2 |
| 2ml | |
3 |
| 1.6 ml | |
4 |
| 100ml | |
5 |
| 100ml | |
6 |
| 11/22 ml | |
7 |
| 11/22 ml | |
8 |
| 15ml | |
9 |
| 2ea/4e | |
10 | microplate serum dilution | 1ea/2e | |
11 | Ntuziaka | 1 pcs |